Regulatory

Part:BBa_K1400003:Design

Designed by: Dylan Siriwardena   Group: iGEM14_uOttawa   (2014-10-06)


pTre(4) Single input tet responsive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 35
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 166


Design Notes

Mig1 sites were scrambled to allow grow up in glucose.


Source

This part was generated by modifying the native Gal promoter from Saccharomyces cerevisiae.

References